DETERMINATION OF THE pKa VALUES OF POLYCYCLIC AROMATIC HYDROCARBON METABOLITES BY CAPILLARY ZONE ELECTROPHORESIS
- 1 January 1999
- journal article
- Published by Taylor & Francis in Journal of Liquid Chromatography & Related Technologies
- Vol. 22 (5) , 669-679
- https://doi.org/10.1081/jlc-100101689
Abstract
Capillary zone electrophoresis (CZE) was used to determine the pKa values of six environmentally and metabolically important PAH metabolites. The pKa values obtained from CZE were close to the pKa values reported in the literature which were obtained by spectroscopy. In this work, it was found that CZE is very suitable for the determination of the pKa values of the weakly acidic, large ring PAH metabolites. A pKa value acquired for one of the model compounds by spectrometry gave supporting evidence for the reliability of CZE in obtaining pKa values for relatively large molecular weight hydroxyl compounds.Keywords
This publication has 23 references indexed in Scilit:
- Separation of polycyclic aromatic hydrocarbon metabolites by γ-cyclodextrin-modified micellar electrokinetic chromatography with laser-induced fluorescence detectionJournal of Chromatography A, 1998
- Separation of hydroxylated polycyclic aromatic hydrocarbons by micellar electrokinetic capillary chromatographyJournal of Chromatography A, 1997
- Simulation of Human Benzo[a]pyrene Metabolism Deduced from the Analysis of Individual Kinetic Steps in Recombinant YeastChemical Research in Toxicology, 1996
- Evaluation of biomarkers in plasma, blood, and urine samples from coke oven workers: significance of exposure to polycyclic aromatic hydrocarbons.Occupational and Environmental Medicine, 1995
- The Liquid Chromatographic Separation of Metabolites of Benzo[a]pyrene with γ-Cyclodextrin as a Mobile Phase AdditiveJournal of Liquid Chromatography, 1995
- Improved high-performance liquid chromatographic method for the determination of polycyclic aromatic hydrocarbon metabolites in human urineJournal of Chromatography B: Biomedical Sciences and Applications, 1995
- Significance of dermal and respiratory uptake in creosote workers: exposure to polycyclic aromatic hydrocarbons and urinary excretion of 1-hydroxypyrene.Occupational and Environmental Medicine, 1995
- Reversed-Phase Chromatographic Separation of Benzo[A]Pyrene Metabolites with β-Cyclodextrin as a Mobile Phase ModifierJournal of Liquid Chromatography, 1995
- Gas chromatographic—mass spectrometric analysis of diols and tetrols from reactions of polycyclic aromatic hydrocarbon epoxides with hemoglobinJournal of Chromatography B: Biomedical Sciences and Applications, 1991
- Metabolism of benzo[a]pyrene and (−)-trans-benzo[a]pyrene-7,8-dihydrodiol by freshly isolated hepatocytes of brown bullheadsChemico-Biological Interactions, 1990