Influence of Three Factors on the Growth and Nutrition of Grapevine Microcuttings

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Abstract
Grapevine microcuttings grown in vitro were subjected to different culture conditions within the framework of a factorial experiment.Three factors were studied: genotype, sucrose content of the medium and physical conditions of culture. There were three values or three different cases for each controlled factor, leading to a total of 27 experimental batches and 15 induced variables were measured. These concerned growth of plants, uptake of carbohydrates from the medium and uptake of essential minerals. Analysis of the results revealed the overall action of each of the three controlled factors. These actions were significant in most cases. At 21°C, genotype and culture conditions influenced length, fresh and dry weight of the plants and the amounts of carbohydrates and nitrate taken up; however, the relative dry matter content of plants remained normal. In contrast, when the greater part of the culture was carried out at 12°C (long-term culture conditions) the percentage dry weight increased considerably.