Inosine Sensor Based on an Amorphous Silicon Isfet
- 1 October 1988
- journal article
- biosensors
- Published by Taylor & Francis in Analytical Letters
- Vol. 21 (10) , 1785-1800
- https://doi.org/10.1080/00032718808066347
Abstract
A sensor for the determination of inosine was prepared by a combination of the enzyme system (shown below) and an amorphous silicon ISFET (a-ISFET). Nucleoside phosphorylase and xanthine oxidase were covalently immobilized on polyvinylbutyral membrane containing 1, 8-diamino-4-aminomethyloctane. The optimum conditions for determination of inosine were pH 7.5, 32[ddot] C. The sensor gave a linear relationship between the initial rate of the output gate voltage change and the logarithm of inosine concentration between 0.02 and 0.1 mM. Determination of inosine was possible within 7 min. The system could be used for two weeks with about 35% loss of enzymatic activity.Keywords
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