Antigenic competition in vitro of spleen cells subjected to a graft-versus-host reaction.

  • 1 August 1971
    • journal article
    • Vol. 21  (2) , 351-61
Abstract
A graft-versus-host reaction was induced in F1 hybrid mice by the inoculation of spleen cells from one of the parental strains. One week later cultures for primary immunization in vitro against sheep red cells were initiated with the spleen cells of the recipients. It was found that the antibody response against sheep red cells was considerably inhibited in spleen cells from mice subjected to a graft-versus-host reaction as compared to the response of spleen cells from untreated mice. Furthermore, when spleen cells from animals subjected to a graft-versus-host reaction were cultured together with spleen cells from normal animals or animals preimmunized to sheep red cells, the antibody responses of the latter two cell populations were inhibited.