DOUBLE ISOTOPE DERIVATIVE ASSAY OF ALDOSTERONE, CORTICOSTERONE, AND CORTISOL
- 1 September 1964
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 42 (9) , 1275-1291
- https://doi.org/10.1139/o64-137
Abstract
A method of double isotope derivative assay of aldosterone, corticosterone, and cortisol is described. It involves acetylation with acetic-3H anhydride of high specific activity followed by the purification of the steroids through four paper chromatographies and the formation of a derivative by chromium trioxide oxidation. The method is particularly suited to the quantitative measurement of these steroids in the incubation media of either rat or beef adrenal slices.Keywords
This publication has 19 references indexed in Scilit:
- IN VIVODISTRIBUTION OF SOME 17-HYDROXYCORTICOSTEROIDS BETWEEN THE PLASMA AND RED BLOOD CELLS OF MAN*Journal of Clinical Endocrinology & Metabolism, 1959
- FUNCTIONAL ZONATION OF THE ADRENAL CORTEX: PATHWAYS OF CORTICOSTEROID BIOGENESISEndocrinology, 1959
- Secretion of Aldosterone by the Zona Glomerulosa of Rat Adrenal Glands Incubated in Vitro.Experimental Biology and Medicine, 1956
- THE PHYSIOLOGICAL DISPOSITION AND METABOLIC FATE OF HYDROCORTISONE IN MANJournal of Clinical Investigation, 1955
- New solvent systems for the resolution of corticosteroids by paper chromatographyArchives of Biochemistry and Biophysics, 1955
- IN VITRO BIOASSAY OF CORTICOTROPIN: MODIFICATION AND STATISTICAL TREATMENTEndocrinology, 1955
- Isonicotinic Acid Hydrazide as Reagent for Determination of Δ4-3-KetosteroidsAnalytical Chemistry, 1955
- Die Konstitution des Aldosterons. Über Bestandteile der Nebennierenrinde und verwandte Stoffe. 92. MitteilungHelvetica Chimica Acta, 1954
- Methods of paper chromatography of steroids applicable to the study of steroids in mammalian blood and tissuesBiochemical Journal, 1952