Insulin‐like growth factor I supports differentiation of cultured osteoblast‐like cells
Open Access
- 23 July 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 173 (1) , 48-52
- https://doi.org/10.1016/0014-5793(84)81015-7
Abstract
Rat calvaria cells grown in culture for one week had properties of osteoblasts: a high content in alkaline phosphatase and a marked cyclic AMP response to parathyroid hormone (PTH). In short‐term experiments, insulin‐like growth factor I (IGFI) stimulated the incorporation of (14C]glucose into glycogen. When IGF I was present in the medium during 6 days the cell number increased slightly and there was a substantial, disproportionate rise in alkaline phosphatase activity of the cultures. Thus, IGFI stimulates growth, and in addition, and in contrast to other growth factors, mainly enhances differentiation of osteoblasts.Keywords
This publication has 14 references indexed in Scilit:
- Stimulation of DNA synthesis by epidermal growth factor in osteoblast-like cellsCalcified Tissue International, 1983
- Insulin-like growth factors stimulate synthesis of nucleic acids and glycogen in cultured calvaria cellsCalcified Tissue International, 1983
- Regulation of Bone FormationNew England Journal of Medicine, 1983
- Regulation of Bone FormationNew England Journal of Medicine, 1983
- The Hormonal and Local Regulation of Bone Formation*Endocrine Reviews, 1983
- Effect of hormones and growth factors on alkaline phosphatase activity and collagen synthesis in cultured rat calvariaeMetabolism, 1983
- Parathormone promotes glycogen formation from [14C]glucose in cultured osteoblast-like cellsFEBS Letters, 1982
- Insulin-like growth factor I stimulates growth in hypophysectomized ratsNature, 1982
- Platelet collection and transfusion using the fenwal CS‐3000 cell separatorTransfusion, 1981
- Bone Cells: A Serum-Free Medium Supports Proliferation in Primary CultureScience, 1978