PHYSICOCHEMICAL DETECTION AND MEASUREMENT OF ALDOSTERONE IN BODY FLUIDS AND TISSUES

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Abstract
The most efficient method for extraction of aldosterone (A) from urine was extraction with chloroform after standing at pH 1 for 24 hours. Fats, if present, were removed by partition between petroleum ether and aqueous ethanol. Final purification was performed on a silica gel column using 1% acetone and chloroform initially and eluting with 50% acetone and chloroform. A was then isolated, using 2 paper chromatographic systems. The 1st system was propylene glycol/toluene and the 2d system was toluene/ethylacetate/methanol/water. Assay of A was done by visual comparison with 17-hydroxycorticosterone using UV light and blue tetrazolium/NaOH. Using this method, the con-tent of A in tissues, blood and various urine samples is given.