Identification of “ Cronobacter ” spp. ( Enterobacter sakazakii )

Abstract
A taxonomic reclassification of the neonatal pathogen Enterobacter sakazakii to consist of five species within a new genus, “ Cronobacter ,” has recently been proposed. The correct identification of these organisms is important to clinicians. Therefore, using 312 Enterobacteriaceae , including 210 “ Cronobacter ” strains, the reliabilities of biochemical and genetic confirmation tests were investigated. All “ Cronobacter ” isolates were positive using dnaG and gluA PCR protocols, and all expressed α-glucosidase activity. ID32E v3.0 identified 99.5% of “ Cronobacter ” isolates (as the nearest match to E. sakazakii ).

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