Special proliferative sites are not needed for seeding and proliferation of transfused bone marrow cells in normal syngeneic mice.

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Abstract
The widely held view that transfused bone marrow cells will not proliferate in normal mice, not exposed to irradiation or other forms of bone marrow ablation, was reinvestigated. Bone marrow cells (4 .times. 107) from male donors were given to female recipients on each of 5 consecutive days, 5-10 times the number customarily used in the past. When the recipients were examined 2-13 wk after the last transfusion, donor cells were found to average 16-25% of total marrow cells. Similar percentages of donor cells were found when variants of the enzyme phosphoglycerate kinase determined electrophoretically were used for identification of donor and recipient cells. The proportion of donor cells is compatible with a linear dependence on the number of cells transfused over the range tested, i.e., 20-200 .times. 106 bone marrow cells injected i.v. Special proliferative sites thus do not appear to be required.