Cyclic regulation of B220 antigen expression in immature B cell lines
- 1 February 1994
- journal article
- Published by Wiley in Immunology & Cell Biology
- Vol. 72 (1) , 75-78
- https://doi.org/10.1038/icb.1994.11
Abstract
Three independent immature B cell lines transformed with temperature-sensitive mutants of Abelson murine leukaemia virus (A-MuLV) persistently expressed B22O antigen detected by both of the anti-B220 antibodies RA3-6B2 and 14.8 during culture at the permissive temperature (35°C). RA3-6B2 recognizes the B220 epitope and that 14.8 is a CD45RA antibody. However, when the culture temperature was shifted up to the non-permissive temperature (39°C) and the culture was continued for 2–3 weeks, a population of the cells (RA3-6B2- 14.8+ cells) that lost RA3-6B2 antigen appeared in all three cell lines. From these populations, RA3-6B2- 14.8 + subclones were independently isolated by limiting dilution and the phenotype was stable during culture at the permissive temperature. When the culture temperature of the RA3-6B2- 14.8 + subbclones was shifted up to the non-permissive temperature and the culture was continued for 2–3 weeks, RA3-6B2 antigen was re-expressed in a subpopulation of the RA3-6B2 - 14.8 + cells. These results demonstrated cyclic regulation of B220 antigen expression during proliferation and/or differentiation of immature B cell lines.Keywords
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