Identification of a novel bifunctional Δ12/Δ15 fatty acid desaturase from a basidiomycete, Coprinus cinereus TD#822‐2
- 2 January 2007
- journal article
- Published by Wiley in FEBS Letters
- Vol. 581 (2) , 315-319
- https://doi.org/10.1016/j.febslet.2006.12.031
Abstract
A new gene encoding a Δ12 fatty acid desaturase-related protein was cloned from a multicellular basidiomycete Coprinus cinereus TD#822-2. The 1326 bp full-length gene, designated as Cop-odeA , codes for a putative protein of 442 amino acids with a MW of 49 224. The Cop-odeA yeast transformant accumulated four new fatty acids identified as 9,12-hexadecadienoic acid, 9,12,15-hexadecatrienoic acid, linoleic acid, and α-linolenic acid, which comprised 8.8%, 1.0%, 29.0%, and 0.6% of the total fatty acids, respectively. The Cop-odeA protein was confirmed to be a novel bifunctional fatty acid desaturase with both high Δ12 desaturase activity and unusual Δ15 desaturase activity. Abbreviations C16:1 palmitoleic acid C16:2 9,12-hexadecadienoic acid C16:3 9,12,15-hexadecatrienoic acid C18:1 oleic acid C18:2 linoleic acid C18:3 α-linolenic acid FAME fatty acid methyl ester GLC gas-liquid chromatography SF subfamily Keywords Δ12 fatty acid desaturase Δ15 fatty acid desaturase Hexadecadienoic acid Hexadecatrienoic acid Coprinus cinereusKeywords
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