Two‐dimensional gel electrophoresis of sheep plasma proteins: Genetic polymorphism of an ai‐protease inhibitor and a post‐transferrin

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Abstract
Two-dimensional electrophoretic analysis of sheep plasma proteins was performed by a 1st-dimension separation in agarose gel (pH 5.0) followed by a 2nd-dimension separation in horizontal polyacrylamide gel (pH 9.0). This method resulted in improved and reproducible separation of many .alpha.- and .beta.-globulins. Two groups of .alpha.1-globulins, designated Pi-1 and Pi-2, were protease inhibitors. These 2 inhibitors differed from each other in protease inhibitory spectra. Genetic polymorphism was observed for the Pi-2 protein and another unidentified protein, tentatively designated as posttransferrin (Ptf). Family data supported the hypothesis that Pi-2 and Ptf types were controlled by codominant, autosomal alleles. Three Pi-2 alleles and 2 Ptf alleles were observed in 1 population of the Gotland breed of sheep. The analysis of data from 50 informative matings showed no evidence of genetic linkage between the Pi-2, Ptf and transferrin (Tf) loci in the population of sheep studied.