Factors Involved in in Vitro Stabilization of Nitrate Reductase from Cotton (Gossypium hirsutum L.) Cotyledons
Open Access
- 1 May 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 61 (5) , 714-717
- https://doi.org/10.1104/pp.61.5.714
Abstract
Experiments were conducted to determine if pretreatment of cotton (Gossypium hirsutum L.) plants resulted in differential in vitro stabilities of nitrate reductase (NR) activity. Although NR activity declines markedly during the second half of the daily light period, in vitro NR stability is not modified by time of harvest. Phenylmethylsulfonylfluoride, iodoacetamide, and N-ethylmaleimide do not influence in vitro NR stability, suggesting that serine or sulfhydryl proteases are not responsible for in vitro lability of NR from cotton cotyledons.This publication has 15 references indexed in Scilit:
- Nitrate Reductase Activity in Maize (Zea mays L.) LeavesPlant Physiology, 1976
- Loss of Ribulose 1,5-Diphosphate Carboxylase and Increase in Proteolytic Activity during Senescence of Detached Primary Barley LeavesPlant Physiology, 1975
- Improvements of the Nitrite Color Development in Assays of Nitrate Reductase by Phenazine Methosulfate and Zinc AcetatePlant Physiology, 1974
- Use of Protein in Extraction and Stabilization of Nitrate ReductasePlant Physiology, 1974
- Anaerobic Nitrite Production by Plant Cells and Tissues: Evidence for Two Nitrate PoolsPlant Physiology, 1973
- In Vivo Assay of Nitrate Reductase in Cotton Leaf DiscsPlant Physiology, 1973
- Synthesis and Turnover of Nitrate Reductase in Corn RootsPlant Physiology, 1972
- Regulation of Nitrate Reductase in Excised Barley RootsPlant Physiology, 1971
- Rapid Changes in Levels of Polyribosomes in Zea mays in Response to Water StressPlant Physiology, 1970
- Some Characteristics of Nitrate Reductase From Higher PlantsPlant Physiology, 1968