Further Studies on the Interaction of Immobilized Folate Binding Protein and Enzyme-Folate Conjugates; An Enzyme-Linked Competitive Binding Assay for Folate
- 1 January 1986
- journal article
- analytical biochemistry-and-clinical-analysis
- Published by Taylor & Francis in Analytical Letters
- Vol. 19 (15-16) , 1653-1678
- https://doi.org/10.1080/00032718608066313
Abstract
The kinetic and thermodynamic properties of the folate interaction with immobilized folate binding protein (FBP) are examined. The use of enzyme-rather than radio-labeled folate provides insight into the complex binding mechanism of folate with FBP and indicates that polymerization of the binding protein (evident for FBP-folate association in solution) is not a prerequisite for the cooperative behavior observed. An enzyme-linked competitive binding assay for folate based on this interaction is described and dose-response curves demonstrate the sensitivity and selectivity of the method. The accuracy of the assay is tested by determining folate in infant formula.Keywords
This publication has 22 references indexed in Scilit:
- Cooperative interaction of immobilized folate binding protein with enzyme-folate conjugates: an enzyme-linked assay for folateAnalytical Chemistry, 1984
- Enzyme immunoassayAnalytical Chemistry, 1984
- Use of enzymes in immunoassay techniques. A reviewThe Analyst, 1984
- Immunoassay and its applications to water analysisTrAC Trends in Analytical Chemistry, 1984
- Immunoassay in therapeutic drug monitoringJournal of Chemical Education, 1983
- Numbers of Receptor Sites from Scatchard Graphs: Facts and FantasiesScience, 1982
- Thermal processing effects on folacin bioavailability in liquid model food systems, liver and cabbageJournal of Agricultural and Food Chemistry, 1982
- Comparison of High Performance Liquid Chromatographic, Radiometric, and Lactobacillus casei Methods for the Determination of Folacin in Selected FoodsJournal of Food Science, 1982
- Reactivation of folate-binding protein from cow's milk, purified by affinity chromatography in the presence of lecithin and other surfactantsBioscience Reports, 1981
- Determination of folacin derivatives in selected foods by high-performance liquid chromatographyJournal of Agricultural and Food Chemistry, 1981