Detection of Thiobacillus ferrooxidans in acid mine environments by indirect fluorescent antibody staining
- 1 July 1976
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 32 (1) , 159-165
- https://doi.org/10.1128/aem.32.1.159-165.1976
Abstract
An indirect fluorescent antibody (FA) staining technique was developed for the rapid detection of Thiobacillus ferrooxidans. The specificity of the FA stain for T. ferrooxidans was demonstrated with both laboratory and environmental samples. Coal refuse examined by scanning electron microscopy exhibited a rough, porous surface, which was characteristically covered by water-soluble crystals. Significant numbers of T. ferrooxidans were detected in the refuse pores. A positive correlation between numbers of T. ferrooxidans and acid production in coal refuse in the laboratory was demonstrated with the FA technique.This publication has 17 references indexed in Scilit:
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