S‐Nitrosoglutathione induces formation of nitrosylmyoglobin in isolated hearts during cardioplegic ischemia — an electron spin resonance study
- 8 January 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 378 (2) , 111-114
- https://doi.org/10.1016/0014-5793(95)01429-2
Abstract
Previously, it has been shown that ·NO donor S‐nitrosoglutathione (GSNO) improves the postischemic functional recovery in crystalloid buffer‐perfused isolated rat hearts subjected to cardioplegic ischemia. Supplementation of cardioplegic solution with nitronyl nitroxide, a scavenger of ·NO, antagonized this protective effect. Using low temperature ESR, we have detected nitrosylmyoglobin (MbNO) in rat hearts subjected to cardioplegic ischemia in the presence of GSNO (20–200 μmol/l). During aerobic reperfusion MbNO signal intensity gradually decreased, but persisted for up to 30 min of aerobic reperfusion. We conclude that MbNO is an endogenous marker of ·NO release in myocardial tissues. Implications of MbNO formation are discussed with respect to cardioprotection during ischemia‐ and reperfusion‐induced myocardial injury.Keywords
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