Sensitive radioimmune assay for measuring Aleutian disease virus antigen and antibody

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Abstract
A solid-phase, 1-step radioimmunoassay was developed which detected as little as 0.02 .mu.l of a standard Aleutian disease virus antigen preparation, .apprx. 3.2 ng of viral protein. Virus antigen was measured in different mink organs and cell types during experimental i.p. infection. The gut and kidney were the 1st organs in which virus antigen was detected (day 3-6 after infection). On day 6 or later, virus antigen was found in spleen, liver, kidney, lymph nodes, peritoneal exudate and bone marrow cells. With inhibition of antigen binding, a radioimmune assay was developed for antibody detection. Viral antibodies were detected as early as 3 days after infection. Antibody titers from 1/105 to > 1/106 were found in plasmacytotic mink. When the sensitivity of the antibody radioimmune assay was compared with that of other known methods for anti-Aleutian disease virus quantitation, the radioimmunoassay was considerably more sensitive, detecting as little as 5 ng of antibody.

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