ISOLATION AND ESTIMATION OF »FREE« OESTROGENS IN HUMAN PLACENTAE

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Abstract
By application of the urinary estrogen method of Brown (Biochem. J. 60: 185, 1955) estrone (E1), estradiol-17[beta] (E2) and estriol (E3) were isolated from placental tissue and were identified by counter-current distribution of various derivatives in several solvent systems. From the analysis of 30 full-term placentae the following geometrical mean values and fiducial limits at the 95% probability level were calculated: E1, 30.8(8.5 - 111.5) [mu]g/kg E2, 128.5 (52.2 - 315.0) [mu]g/kg; and E3, 227.8 (50.2 - 1030.0) [mu]g/kg. Recoveries varied from an average of 60% for E1 to 75% for E2. Following correction for these losses the mean concentrations of the estrogen in full-term placentae then become: E1, 51.3 (14.2 - 185.8) [mu]g/kg; E2, 170.2 (69.5 - 417.2) [mu]g/kg; and E3, 314.6 (69.3 - 1423.0) [mu]g/kg wet tissue.