Cacao polyphenolic substances. 3. Separation and estimation on paper chromatograms

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Abstract
The application of two-way and quantitative paper chromatography to the polyphenols of the cacao bean is shown. The bean contain 4 catechins (of which 92% is (-)-epicatechin), and at least 3 leucocyanidin compounds and 2 cyanidin glycosides. Some 3% of catechins and 2.5% of leuco-cyanidins are present in the freshly dried bean. Separation of the main leukocyanidin from the other polyphenols was followed.