Antigenic Differences between the Membrane Antigen Polypeptides Determined by Different EB Virus Isolates

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Abstract
The antigenic specificities of the membrane antigen (MA) complex on 3 Epstein-Barr (EB) virus-producing cell lines were compared by complete absorption of an anti-MA antiserum with P3HR-1 cells [human lymphoblastoid], followed by testing for residual anti-MA antibody activity against B95-8 [marmoset lymphoblastoid] and QIMR-WIL [human lymphoblastoid] cells. Indirect membrane immunofluorescence showed that the absorbed serum, which had lost the capacity to bind P3HR-1 cells, gave bright staining on a small proportion of B95-8 cells SDS(sodium dodecyl sulfate)-polyacrylamide gel electrophoretic analysis of 125I-labeled MA polypeptides showed that the absorbed serum was able to isolate MA polypeptides from B95-8 and QIMR-WIL cells but not from P3HR-1 cells. There is substantial sharing of antigenic specificities between the MA determined by 3 isolates of EB virus and some antigenic divergence.

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