Metabolism of Human Brain Cortex in Vitro

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Abstract
The endogenous carbohydrate content of human cerebral cortex slices did not account for the total O2 consumption observed during a 3 hour study of endogenous metabolism. The amount of glucose which is taken up in the presence of 0.01 [image] glucose is in excess of that required to support respiration. Experiments with 0.01 [image] radioactive glucose indicate that the glucose taken up is oxidized to some extent, converted to lactic acid and assimilated and/or stored in the tissue. Although experiments indicated that glucose oxidation increased as endogenous metabolism decreased, the amount of radioactive glucose oxidation accounted for only approximately 50% of the total 02 consumption in the presence of glucose during a 3 hour study. Lactic acid produced in the presence of extracellular glucose is derived exclusively from exogenous glucose via the Embden-Meyerhof scheme. Furthermore it appears that lactic acid accumulates extracellularly in the presence of glucose only as long as there is an excess amount of intracellular glucose during considerable concomitant endogenous metabolism. Aerobic lactic acid accumulation increases when glucose is present in combination with pyruvate or gluta-mate but not with alanine.

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