Flavin-adenine-dinucleotide in rat tissues

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Abstract
The flavin-adenine-dinucleotide content of boiled extracts of brain, heart, kidney and liver tissue of normal rats was detd. There was a decrease in the flavin-adenine-dinucleotide content of boiled extracts of liver and heart tissue from rats fed on a flavin-deficient diet. No significant difference could be detected in extracts of brain and kidney tissue. Inj. of 1 mg. lactoflavin, 24.4 and 1/2 hr- before death restored the flavin-adenine-dinucleotide content of liver and kidney tissue of flavin-deficient rats to normal levels. This constituted an in vivo synthesis of the nucleotide from lactoflavin. The restoration was more rapid in the liver than in the heart. There was no significant difference between the flavin-adenine-dinucleotide contents of boiled extracts of tissues from normal and adrenalectomized rats. There was no flavin-adenine-dinucleotide activity in unheated blood (ox, rat), but this activity was present after heating the blood at 100[degree] for 3 min. at pH 7. The flavin-adenine-dinucleotide in the blood of flavin-deficient rats was lower than that of normal animals, but was restored by inj. of 1 mg. lactoflavin 24, 4, 1/2 hrs. before death. The enzymic breakdown of the flavin-adenine-dinucleotide in the tissues was found to be dependent upon pH. Breakdown was more rapid with liver than with brain tissue.