Studies of human alloantigens on man‐mouse hybrids: possible syntheny between hl‐a and p systems

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Abstract
The expression of HL‐A, P and H (ABO) systems has been studied on man‐mouse hybrid clones and their subclones. Using the microcomplement fixation technique, we detected antigens of the HL‐A, P and H systems in some clones corresponding to the phenotype of the human cell donor. Absorption tests were used to provide the specificity of the alloantisera. We also found a positive correlation between the presence of HL‐A and P1/P antigens in these clones, but no correlation was found between HL‐A and Pk specificities and between HL‐A and H specificities. It was thought that the positive correlation might be due to a syntheny between HL‐A and P loci or to the presence of HL‐A and P specificities on a unique molecule. Therefore, preliminary experiments were made using antibody‐induced redistribution phenomena (capping) to study the relationships between P and HL‐A antigens at the cell surface. They indicated that the two molecules are independent of one another.