Sequence-dependent structural variations of hammerhead RNA enzymes
Open Access
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (5) , 1103-1108
- https://doi.org/10.1093/nar/18.5.1103
Abstract
The discovery of in vivo catalytic activity for the hammerhead RNA self-cleaving domain has led to the development of a new class of sequence-specific RNA endonucleases. Two such ribozymes have been synthesized using in vitro transcription with T7 polymerase and their structures have been studied by optical spectroscopy, nuclear magnetic resonance and nondenaturing gel electrophoresis. These data show the presence of a stable hairpin consisting of a double helical stem and a tetranucleotlde loop in both RNA enzymes. Additional structure, with different stabilities, Is also observed in both RNA enzymes. The half-lives for cleavage of the complementary RNA substrates by these two RNA enzymes have been previously shown to differ by a factor of 50. The data presented here suggest that this rate difference may be a result of the formation of catalytically inactive conformations In the RNA enzyme which Interfere with formation of the enzyme-substrate complex.This publication has 16 references indexed in Scilit:
- A catalytic 13-mer ribozymeNucleic Acids Research, 1989
- Simple RNA enzymes with new and highly specific endoribonuclease activitiesNature, 1988
- Construction of a series of several self‐cleaving RNA duplexes using synthetic 21‐mersFEBS Letters, 1988
- A small catalytic oligoribonucleotideNature, 1987
- Self-cleavage of virusoid RNA is performed by the proposed 55-nucleotide active siteCell, 1987
- The Chemistry of Self-Splicing RNA and RNA EnzymesScience, 1987
- Self-cleavage of plus and minus RNAs of a virusoid and a structural model for the active sitesCell, 1987
- Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templatesNucleic Acids Research, 1987
- Structural and Ionic Requirements for Self-cleavage of Virusoid RNAs and trans Self-cleavage of Viroid RNACold Spring Harbor Symposia on Quantitative Biology, 1987
- Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymenaCell, 1982