Ac-Globulin (Factor V): Preparation of a Practical Product
- 1 January 1972
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 2 (4) , 381-396
- https://doi.org/10.1080/00327487208065675
Abstract
A procedure for the preparation of large quantities of very potent, high yield, partially pure bovine Ac-globulin concentrate, free of other clotting factors, is described. The isolation procedure involves a plasma recalcification step followed by ion exchange chromatography on a fully quaternized, amino ethyl cellulose. The resulting practical product is stable and represents virtually 100% yield with a purification of over 2000 times from bovine adsorbed plasma.Keywords
This publication has 11 references indexed in Scilit:
- Effect of proteolytic enzymes on bovine factor V. I. Kinetics of activation and inactivation by bovine thrombinBiochemistry, 1969
- The isolation of factor V from bovine plasmaBiochemical Journal, 1967
- Purification and Stabilization of Factor VNature, 1963
- ProthrombinPublished by Harvard University Press ,1962
- A One-Stage Method for the Determination of Accelerator Globulin.Experimental Biology and Medicine, 1953
- Two-stage Procedure For the Quantitative Determination of Prothrombin Concentration*American Journal of Clinical Pathology, 1949
- The Function of Ac-Globulin in Blood ClottingScience, 1947
- Natural Formation of Petroleum-like Hydrocarbons From "Oil Shales"Science, 1947
- ON THE QUANTITATIVE RELATIONSHIP BETWEEN CALCIUM AND PROTHROMBINAmerican Journal of Physiology-Legacy Content, 1947
- Acceleration of Thrombin Formation by a Plasma ComponentNature, 1946