Cytological Detection of Parathyroid Hormone by Immunofluorescence

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Abstract
Summary The fluorescent antibody tech™ nic has been utilized to detect and to localize parathyroid hormone in the chief cells of bovine, human and rat parathyroid tissue. Im-munochemical cross-reaction has been found among these species. The oxyphil cells of human parathyroid tissue did not reveal specific fluorescence. This observation is regarded as further evidence that the oxyphil cells do not normally synthesize or store parathyroid hormone. The authors are indebted to Mr. Clarence Sidwell and Mr. Lemuel Hall of the Research Laboratory for technical assistance; to Mrs. Patricia Boulware and Dr. K. Using Wu of the Pathology Service for assistance in tissue preparation and for histologic consultation; and to the Medical Illustration Service for preparing the photographs.