TagCleaner: Identification and removal of tag sequences from genomic and metagenomic datasets
Open Access
- 23 June 2010
- journal article
- Published by Springer Nature in BMC Bioinformatics
- Vol. 11 (1) , 341
- https://doi.org/10.1186/1471-2105-11-341
Abstract
Sequencing metagenomes that were pre-amplified with primer-based methods requires the removal of the additional tag sequences from the datasets. The sequenced reads can contain deletions or insertions due to sequencing limitations, and the primer sequence may contain ambiguous bases. Furthermore, the tag sequence may be unavailable or incorrectly reported. Because of the potential for downstream inaccuracies introduced by unwanted sequence contaminations, it is important to use reliable tools for pre-processing sequence data.Keywords
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