Correlation between Photosynthetic Activity and Membrane Integrity in Isolated Pea Chloroplasts
Open Access
- 1 November 1965
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 40 (6) , 1157-1161
- https://doi.org/10.1104/pp.40.6.1157
Abstract
Pea chloroplasts, isolated in sucrose media were examined under the phase contrast microscope. The proportion of otherwise entire chloroplasts which showed the granular appearance characteristic of membrane loss varied between 10% and 50% in in individual preparations. Exposure to hypotonic media was followed by immediate membrane loss. Membrane-free chloroplasts showed an enhanced ability to catalyse cyclic photophosphorylation and a reduced ability to catalyse carbon dioxide fixation. Expressed as a fraction of the rate achieved by membrane-free chloroplasts, photophosphorylation by chloroplasts with intact membranes varied between 12% and 65%.This publication has 9 references indexed in Scilit:
- Light and Dark C14O2 Fixation by Spinach Leaf SystemsPlant Physiology, 1965
- Improved Rates of Carbon Dioxide Fixation by Illuminated ChloroplastsBiochemical Journal, 1964
- The isolation of structurally intact chloroplastsBiochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects, 1964
- The cytochromes of isolated chloroplastsProceedings of the Royal Society of London. B. Biological Sciences, 1964
- Uncoupling of the hill reaction from photophosphorylation by anionsArchives of Biochemistry and Biophysics, 1962
- Role of Hexose Monophosphate Pathway in Tomato CatabolismPlant Physiology, 1962
- Pyocyanine and Phosphorylation with Chloroplasts.Plant Physiology, 1959
- COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARISPlant Physiology, 1949
- The estimation of phosphorusBiochemical Journal, 1940