REGULATION OF TIMOTHY GRASS-POLLEN IGE ANTIBODY-FORMATION .1. INVITRO INDUCTION OF SUPPRESSOR T-CELLS BY SOLUBLE T-SUPPRESSOR FACTORS

  • 1 January 1983
    • journal article
    • research article
    • Vol. 49  (3) , 463-469
Abstract
A method using mini-Marbrook chambers in culturing normal [mouse] spleen cells with timothy-specific T suppressor factor (TSF) and 2nd order T suppressor factor (TSF2) to induce suppressor T (Ts) cells is described. Spleen cells cultured with T suppressor factors injected i.v. into recipients primed 20 days earlier with antigen and boosted with antigen within 24 h of cell transfer, significantly suppress a secondary anti-antigen-B (AgB)-IgE response. Spleen cells cultured with normal rabbit IgG failed to suppress the secondary anti-AgB-IgE response in recipient mice. The failure of enriched T cells cultured with either TSF or TSF2 to produce TS cells suggests that an accessory cell may be important in presentation of these soluble factors during the induction of TS cells.