NUTRITION OF ANIMAL CELLS IN TISSUE CULTURE: VII. USE OF REPLICATE CELL CULTURES IN THE EVALUATION OF SYNTHETIC MEDIA

Publisher Website
Google Scholar
Abstract
The replicate culture assay procedures of Earle and his associates were adapted for use in evaluating effectiveness of synthetic media. For this purpose, use was also made of Earle''s L strain mouse cells. Washed and continuously stirred suspensions of these or similar strains of cells may be dispensed, with reasonable assurance of uniformity, into a series of replicate cultures, the number depending on the volume of suspension and the capacity and effectiveness of the stirring and dispensing unit. For use with synthetic media, the original procedures for prepn. and care of the replicate cultures and for their subsequent treat-ment for counting of isolated, stained nuclei were modified considerably. This paper describes the procedures finally adopted and also describes a relatively simple screening procedure in which washed cell suspensions may be used to advantage in making preliminary assays of synthetic media and in testing the relative toxicity or growth stimulating effects of substances added to, or derived from, natural media.