NUTRITION OF PASTEURELLA PESTIS IN CHEMICALLY DEFINED MEDIA AT TEMPERATURES OF 36 TO 38 C

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Abstract
The requirements for growth of 14 strains of P. pestis at 36 to 38[degree]C were established. All strains required insoleucine, valine, threonine, phenylalanine, methionine, and cysteine. The requirement for hemin was satisfied by a-ketoglutaric acid or large amounts of glutamic acid, but some evidence indicated that an impurity in the latter may have been responsible for its activity. Biotin was required at 36[degree]C and above, and thiamine and pantothenate became necessary at 36[degree]C and above, and thiamine and pantothenate became necessary at 38[degree]C.