Cloning and chromosomal localization of Drosophila cDNA encoding the catalytic subunit of protein phosphatase 1α
- 1 August 1989
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 183 (3) , 603-610
- https://doi.org/10.1111/j.1432-1033.1989.tb21089.x
Abstract
A 1.2-kb clone containing the full coding sequence of a protein phosphatase 1 catalytic subunit has been isolated from a Drosophila head cDNA library. It encodes a polypeptide of 302 amino acids with a molecular mass of 34.5 kDa. The predicted sequence is 92% identical (94% similar) to rabbit protein phosphatase 1.alpha. (PP-1.alpha.) demonstrating strict conservation of the phosphatase catalytic subunit over a considerable evolutionary distance. Abundant 1.6-kb and 2.5-kb mRNA transcripts were detected throughout Drosophila development. The clone hybridised to four sites on Drosophila salivary gland polytene chromosomes. The major site is at 87B6-12 on the right arm of chromosome 3. In addition, there are three secondary sites, one on the same chromosome at 96A2-5 and two on the X chromosome at 9C1-2 and 13C1-2. Isolation of a further cDNA clone, hybridising to 9C1-2 and encoding part of the catalytic subunit 88% similar to Drosophila PP-1.alpha., proves the existence of at least two transcriptionally active genes for protein phosphatase 1.This publication has 44 references indexed in Scilit:
- Identification of a novel protein phosphatase catalytic subunit by cDNA cloningFEBS Letters, 1988
- Two isoforms of protein phosphatase 1 may be produced from the same geneFEBS Letters, 1988
- A second catalytic subunit of type‐2A protein phosphatase from rabbit skeletal muscleFEBS Letters, 1987
- Isolation and sequence analysis of a cDNA clone encoding a type‐1 protein phosphatase catalytic subunit: Homology with protein phosphatase 2AFEBS Letters, 1987
- Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of a type‐2A protein phosphataseFEBS Letters, 1987
- Structure and sequence of the Drosophila melanogaster calmodulin geneJournal of Molecular Biology, 1987
- Purification of the catalytic subunit of protein phosphatase-1 from Drosophila melanogasterBiochemical and Biophysical Research Communications, 1987
- Identification of high levels of protein phosphatase‐1 in rat liver nucleiFEBS Letters, 1986
- Protein Import into the Cell NucleusAnnual Review of Cell and Developmental Biology, 1986
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982