IDENTIFICATION OF THE ANTIDIURETIC SUBSTANCE IN HUMAN URINE

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Abstract
1. The urine of human subjects voided after smoking has been extracted by a method employing adsorption on zinc ferrocyanide and the extracts tested for the antidiuretic activity of arginine vasopressin by the intravenous assay method of Jeffers et al. with modifications by Dicker. 2. Smokers'' urine extracts had an antidiuretic activity of approximately 100 mU of arginine vasopressin per litre of urine. 3. The pattern of the antidiuretic response of the urine extracts with regards to the dose-response relationship and duration of action was identical with that elicited by the U.S. P. reference standard of arginine vasopressin. 4Upon paper chromatography by the descending method with the butanol-acetic acid-water system, the urine extract showed an Rf value identical to that of another sample of arginine vasopressin. 5The antidiuretic substance excreted in human smokers'' urine is arginine vasopressin, the hormone from the posterior lobe of the pituitary gland.