Protein kinase C during differentiation of human promyelocytic leukemia cell line, HL‐60
- 9 April 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 263 (1) , 31-34
- https://doi.org/10.1016/0014-5793(90)80698-i
Abstract
Protein kinase C (PKC) from human promyelocytic leukemia HL‐60 cells can be resolved into three fractions (peak a, b and c) by hydroxyapatite column chromatography. Peak a and c enzymes are indistinguishable from the brain type II PKC having β (βI and βII)‐sequence and type III having α‐sequence, respectively. Peak b enzyme is a previously unidentified PKC subspecies that has enzymological properties subtly different from type I (having γ‐sequence), type II and type III PKC. Upon treatment of HL‐60 cells with 1 μM retinoic add, this peak b enzyme is decreased dramatically within 24 h, whilst peak a enzyme (β‐PKC) is increased, and peak c (α‐PKC) enzyme is slightly decreased within 48 h. The result implies that the PKC subspecies in HL‐60 cells have distinct functions during cell differentiation.Keywords
This publication has 16 references indexed in Scilit:
- THE PROTEIN KINASE C FAMILY: HETEROGENEITY AND ITS IMPLICATIONSAnnual Review of Biochemistry, 1989
- Protein kinase C zeta subspecies from rat brain: its structure, expression, and properties.Proceedings of the National Academy of Sciences, 1989
- The molecular heterogeneity of protein kinase C and its implications for cellular regulationNature, 1988
- A novel phorbol ester receptor/protein kinase, nPKC, distantly related to the protein kinase C familyCell, 1988
- Identification of three additional members of rat protein kinase C family: gd‐, ϵ‐ and ξ‐subspeciesFEBS Letters, 1987
- Protein Kinase C Contains a Pseudosubstrate Prototope in Its Regulatory DomainScience, 1987
- Immunochemical identification of protein kinase C isozymes as products of discrete genesBiochemical and Biophysical Research Communications, 1987
- Identification of the structures of multiple subspecies of protein kinase C expressed in rat brainFEBS Letters, 1987
- Translocation of protein kinase C in human leukemia cells susceptible or resistant to differentiation induced by phorbol 12-myristate 13-acetate.Proceedings of the National Academy of Sciences, 1986
- Calcium‐activated, phospholipid‐dependent protein kinase activity and protein phosphorylation in HL60 cells induced to differentiate by retinoic acidFEBS Letters, 1985