Excretion of Hydrogen Peroxide in Human Urine

Publisher Website
Google Scholar
Abstract
The excretion of hydrogen peroxide in human urine has been demonstrated for the first time. This was accomplished by a new radioactive method developed on the basis of decarboxylation of alpha-ketoglutaric acid by H2 O2. Urine samples were incubated with alpha ketoglutarate pulsed with iwts 1 -14C-analogue, and CO2 formed by decarboxylation was determined by radioactivity measurements. Blanks were prepared by pre-incubation of the samples with catalase. Both male and female subjects were studied. On an average the concentration of H2 O2 in urine was approximately 100 ± 60 μM(10-4 M) Peroxide excretion was found to be unexpectedly high and might thus be useful for clinical diagnosis and therapy in diseases purported to be related to oxidative stress.