Dynamic Fluorogenic Labelling of Pesticides for High Performance Liquid Chromatography: Detection of N-Methylcarbamates with o-Phthalaldehyde

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Abstract
A post-column fluorogenic labeling reaction between o-phthalaldehyde (OPA) and methylamine, a hydrolysis product of the N-methylcarbamate and carbamoyl oxime pesticides [Baygon, Lannate, Temik, Matacil, Mesurol, carbofuran and carbaryl (insecticides)], is described, which provides a highly sensitive and selective detection mechanism for the high performance liquid chromatography of these pesticides. Separations were achieved by reverse phase, with solvent programming of water-dioxane mixtures. As little as 1 ng of the pesticides could be detected, with the last eluting carbamate, Mesurol, requiring 22-54 min, depending upon column and program contour. Chromatography was performed with a modular instrument and with a commercially available fully cabineted instrument.