Quantitative telomeric overhang determination using a double-strand specific nuclease
Open Access
- 21 November 2007
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 36 (3) , e14
- https://doi.org/10.1093/nar/gkm1063
Abstract
Telomeres terminate in 3′ overhangs that function in end protection and the formation of t-loops. Determining the steps and factors involved in overhang processing is compromised by the inability to easily and accurately determine overhang size in the presence of many kilobases of double-stranded telomeric DNA. We here describe the use of a double-strand specific nuclease (DSN) that entirely digests double-stranded DNA including telomeres, leaving the overhangs intact so that they can be measured.Keywords
This publication has 16 references indexed in Scilit:
- Renaturation, activation, and practical use of recombinant duplex-specific nuclease from Kamchatka crabBiochemistry (Moscow), 2006
- Human Telomeres Have Different Overhang Sizes at Leading versus Lagging StrandsMolecular Cell, 2006
- G-tail telomere HPA: simple measurement of human single-stranded telomeric overhangsNature Methods, 2005
- Human Telomeres Maintain Their Overhang Length at SenescenceMolecular and Cellular Biology, 2005
- Protection of mammalian telomeresOncogene, 2002
- Switching and Signaling at the TelomereCell, 2001
- The length of telomeric G-rich strand 3'-overhang measured by oligonucleotide ligation assayNucleic Acids Research, 2001
- Normal human chromosomes have long G-rich telomeric overhangs at one endGenes & Development, 1997
- The terminal DNA structure of mammalian chromosomesThe EMBO Journal, 1997
- Long G Tails at Both Ends of Human Chromosomes Suggest a C Strand Degradation Mechanism for Telomere ShorteningCell, 1997