DIE BESTIMMUNG DES DEHYDROEPIANDROSTERONS IM HARN

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Abstract
A butanol extract of urine was hydrolyzed with dilute acid or with beef liver sulfatase. Crude extracts of 11 normal urines were separated into ketonic and non-ketonic fractions. The ketonic fraction was further subdivided by digitonin precipitation into [alpha]- and [beta]-fractions. Zimmerman (17-KS) and Pettenkofer (DHA) chromagens were determined on both the [alpha]- and [beta]-fractions. By extraction of the Pettenkofer color with ethylene chloride clear blue colors were obtained. Even so there was as much or more Pettenk ofer color in the [alpha]-fraction as in the [beta]-fraction. Sulfatase hydrolysis produced almost as much 17-keto-steroid as dilute acid hydrolysis. Both methods yielded about 50% of the 17-KS produced by strong acid hydrolysis.