Einfluß des Sauerstoffpartialdrucks auf die Citronensäuresynthese durchSaccharomycopsis lipolytica ausn-Paraffinen

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Abstract
The dependence of growth formation of citric acids (citrate: isocitrate = 1:1) on oxygen parital pressure of an alkane utilising yeast Saccharomycopsis lipolytica was investigated. During growth oxygen corresponds to a Michaelis-Menten-kinetics (Ks = 2.0 . 10(-5) M). The respiration quotient RQ for a dissolved oxygen concentration in the range of 10-100% (air saturation) is 0.46 +/- +/- 0.04. The phase of product formation is characterized by 3 sections. Immediately after N-exhaustion the cell activities are the highest. They decline during the first 30 hours of production. Besides the production of reserve material in this first section the highest production rate for citrate and isocitrate is observed. The rate of citric acid production depends on the oxygen partial pressure and is governed by Michaelis-Menten-kinetics. The specific production rate and the rate of oxygen consumption correspond to KS-values of 4.0 X 10(-5) and 3.3 X 10(-5) M, respectively. The RQ-value declines to a constant value of 0.23 +/- 0.02 and is not influenced by oxygen partial pressures in the range of 10--100% (related to air saturation). During the second section cell activities remain nearly constant for about 100 h. Due to this constancy the following equation could be derived: 14 O2 + C15H32 leads to 2 C6H8O7 + 3 CO2 + 8 H2O. In the third section the cell activities decline again.