Nucleotide sequence of the 5′-terminal coding region for pre-rRNA and mature 17S rRNA inTetrahymena thermophilarDNA
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (2) , 959-972
- https://doi.org/10.1093/nar/12.2.959
Abstract
The 5'-terminus of 35S pre-rRNA and mature 17S rRNA of Tetrahymena thermophila was mapped on cloned rDNA fragments by S1 nuclease protection experiments. A single site for transcription initiation was observed when pre-rRNA prepared by three different methods was used as RNA probe. These mapping results were unambiguously confirmed by sequencing the 5'-terminal region of in vitro capped 35S pre-rRNA. DNA sequence analysis of about 520 nucleotides upstream of the transcription initiation site revealed several distinct sets of highly conserved repeat sequences. In addition, the 840 nucleotides downstream of the transcription initiation site (+ 1) was determined and shown to include the 5'-terminus of the 17S rRNA coding region at position + 647. A region surrounding the position + 195 contains an inverted repeat sequence which could be the structural basis for the recently described premature transcription termination event in this organism (Kister et al. (1983) Nucl. Acids Res. 11, 3487-3502).Keywords
This publication has 42 references indexed in Scilit:
- A transcriptional function for the repetitive ribosomal spacer in Xenopus laevisNature, 1983
- Variety in the level of gene control in eukaryotic cellsNature, 1982
- Nucleotide sequence of the transcription initiation region of a rat ribosomal RNA geneGene, 1982
- In vivo sequence requirements of the SV40 early promoter regionNature, 1981
- Transcriptional control regions of the adenovirus VAI RNA geneCell, 1980
- Detailed transcription map of the extrachromosomal ribosomal RNA genes in Tetrahymena thermophilaJournal of Molecular Biology, 1980
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3′ border of the regionCell, 1980
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5′ border of the regionCell, 1980
- Isolation of a high specific activity 35S ribosomal RNA precursor from Tetrahymena pyriformis and identification of its 5' terminus, pppApBiochemistry, 1978
- 5'- and 3'-Terminal nucleotide sequences of Tetrahymena pyriformis 17S rRNABiochemistry, 1977