Evidence that chlorpromazine and prostaglandin E1 but not neomycin interfere with the inositol phospholipid metabolism in intact human platelets
- 7 May 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 264 (1) , 33-36
- https://doi.org/10.1016/0014-5793(90)80757-a
Abstract
Human platelets that had been prelabelled with [32P]Pi were stimulated with trombin in the presence or absence of neomycin, prostaglandin E1 (PGE1) or chlorpromazine. The content of [32P]Pi in phosphatidylinositol 4‐phosphate, phosphatidylinositol 4,5‐bisphosphate and phosphatidic acid (PA) were determined. The data demonstrate that PGE1 and chlorpromazine but not neomycin interfere with the tight metabolic relationship that exists between the inositol phospholipids and PA in thrombin‐stimulated platelets [(1989) Biochem. J. 263, 621‐624]. Our results therefore indicate that neomycin does not inhibit signal transduction in intact platelets at the level of the inositol phospholipid metabolism.Keywords
This publication has 15 references indexed in Scilit:
- Evidence for tight metabolic control of the receptor-activated polyphosphoinositide cycle in human plateletsBiochemical Journal, 1989
- Inositol Trisphosphate, Calcium, Lithium, and Cell SignalingPublished by American Medical Association (AMA) ,1989
- Neomycin inhibits agonist-stimulated polyphosphoinositide metabolism and responses in human plateletsBiochemical and Biophysical Research Communications, 1987
- Phosphorylation of phosphoinositides in human plateletsThrombosis Research, 1986
- Differential effects of chlorpromazine on secretion, protein phosphorylation and phosphoinositide metabolism in stimulated plateletsBiochemical Journal, 1986
- Agents that elevate platelet cAMP stimulate the formation of phosphatidylinositol 4‐phosphate in intact human plateletsFEBS Letters, 1986
- Thin-layer chromatography of polyphosphoinositides from platelet extracts: Interference by an unknown phospholipid.Thrombosis Research, 1985
- Role of guanine nucleotide binding protein in the activation of polyphosphoinositide phosphodiesteraseNature, 1985
- The cytoplasmic concentration of free calcium in platelets is controlled by stimulators of cyclic AMP production (PGD2, PGE1, forskolin)Biochemical and Biophysical Research Communications, 1983
- INHIBITION BY NEOMYCIN OF POLYPHOSPHOINOSITIDE TURNOVER IN SUBCELLULAR FRACTIONS OF GUINEA‐PIG CEREBRAL CORTEX IN VITROJournal of Neurochemistry, 1976