EVALUATION OF DIFFERENT ANTIGENS IN THE COMPLEMENT-FIXATION TEST FOR DIAGNOSIS OF HAEMOPHILUS-PLEUROPNEUMONIAE (PARAHAEMOLYTICUS) INFECTIONS IN SWINE
- 1 January 1979
- journal article
- research article
- Vol. 40 (11) , 1564-1567
Abstract
The identification of new serotypes of H. pleuropneumoniae (parahaemolyticus) and the frequency of pleural adhesions due to contagious pleuropneumonia in many fattening swine herds have prompted the study of the complement-fixation (CF) test as a diagnostic tool for use in swine. Whole cell antigens [Ag], mixed Ag, autoclaved Ag and phenol-water-extracted Ag derived from different serotypes were prepared and tested with immunized-swine sera by the CF test. Mixed Ag consisting of whole cells from all known serotypes was the best screening Ag for routine use. This Ag gave positive titers with all sera in which a positive reaction against the separate serotype Ag was registered. The most highly serotype-specific reactions were obtained with Ag prepared by phenol-water extractions of whole cells. When whole-cell Ag were used in the CF test, antibodies to superficial serotype-specific and common species-specific Ag could be detected.This publication has 7 references indexed in Scilit:
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