Cloning in Escherichia coli and physical structure of hepatitis B virion DNA.
Open Access
- 1 May 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (5) , 2222-2226
- https://doi.org/10.1073/pnas.76.5.2222
Abstract
A restriction map of hepatitis B virion DNA was established after cloning of the whole viral genome in Escherichia coli. By use of EcoRI, Xho I, Bgl II, Xba I, BamHI, HincII, and Hae III endonucleases, a total of 28 restriction sites were mapped. The single-stranded region was localized on the restriction map and 5' end of the short strand was mapped at a fixed position.This publication has 17 references indexed in Scilit:
- A rapid method for determining sequences in DNA by primed synthesis with DNA polymerasePublished by Elsevier ,2004
- Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter controlMolecular Genetics and Genomics, 1979
- THE GENOME OF HEPATITIS B VIRUSAnnual Review of Microbiology, 1977
- Australia Antigen and the Biology of Hepatitis BScience, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- Genome of hepatitis B virus: restriction enzyme cleavage and structure of DNA extracted from Dane particles.Proceedings of the National Academy of Sciences, 1975
- Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresisBiochemistry, 1975
- VIRUS-LIKE PARTICLES IN SERUM OF PATIENTS WITH AUSTRALIA-ANTIGEN-ASSOCIATED HEPATITISThe Lancet, 1970
- Nucleotide Sequence from the Coat Protein Cistron of R17 Bacteriophage RNANature, 1969