A clinical trial on a plate? The potential of 384-well format solid phase extraction for high-throughput bioanalysis using liquid chromatography/tandem mass spectrometry
- 13 December 2000
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 15 (1) , 33-40
- https://doi.org/10.1002/1097-0231(20010115)15:1<33::aid-rcm188>3.0.co;2-4
Abstract
The application of 384‐well format solid phase extraction (SPE) for bioanalysis using liquid chromatography/tandem mass spectrometry (LC/MS/MS) is reported and a 384‐well SPE method for the 5‐HT agonist sumatriptan in human plasma described. Plasma samples were extracted on a prototype low‐density polyethylene 384‐well SPE block using a packed bed of 5 mg Oasis™ HLB. Liquid handling was automated by a combination of a robotic sampler processor and a 96/384 multi‐channel dispensing station. Samples and SPE reagents were drawn through the SPE block by centrifugation. The extracts were analysed by LC/MS/MS with thermally and pneumatically assisted electrospray ionisation and selected reaction monitoring. The method is used to illustrate and discuss the feasibility and viability of sample preparation techniques in high‐density microtitre plate format for routine bioanalysis. Copyright © 2000 John Wiley & Sons, Ltd.Keywords
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