INTERACTION OF SUB-POPULATIONS OF MURINE LYMPH-NODE LYMPHOCYTES IN ANTIGEN-INDUCED [THYMIDINE-C-14 INCORPORATION - T-CELL AND B-CELL SYNERGY IN THE RESPONSE TO ANTIGEN

  • 1 January 1979
    • journal article
    • research article
    • Vol. 38  (2) , 301-310
Abstract
The antigen-induced [14C]-thymidine incorporation of murine lymph node cells (LNC) that were non-adherent (NAD) or adherent (AD) to nylon wool was studied. In contrast to NAD-LNC, AD-LNC responded like unfractionated LNC and these responses were T [thymus-derived] lymphocyte dependent. By co-culturing NAD-LNC with subpopulations of AD-LNC the cellular requirements and interactions necessary for maximal incorporation of [14C]-thymidine were determined. A synergistic effect was observed when NAD-LNC and AD-LNC were co-cultured. Synergism was not dependent on T lymphocytes or macrophages in the AD-LNC population but was associated with the B [bone marrow-derived] lymphocyte subpopulation. The number of B lymphocytes present in a population of LNC can significantly influence the magnitude of the response to antigen.

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