TRANSPLANTATION IN MINIATURE SWINE .5. CHARACTERIZATION OF IA ANTIGENS

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 122  (2) , 623-627
Abstract
The complexity of the I region analog associated with the MHC [major histocompatibility complex] of miniature swine (MSLA) was probed by sequential antibody precipitation studies of Ia [immune response-associated] antigens [Ag]. Treatment of solubilized lymphocyte preparations from MSLA homozygotes of the DD haplotype with excess AA anti-DD alloantiserum led to precipitation of only a portion of the total Ia Ag, as determined by secondary precipitation of the remaining material with CC anti-DD serum. The presumed I region of miniature swine must code for more than 1 Ia Ag-bearing polypeptide chain. Certain mouse alloantisera that react with rat Ia Ag were tested for reactivity with swine Ia Ag. Anti-Iak mouse alloantisera precipitated Ia molecules from every swine extract tested, regardless of MHC type, precluding genetic mapping studies. Sequential precipitation studies demonstrated that the cross-reactive mouse alloantisera reacted only with a subclass of swine Ia Ag, again suggesting genetic complexity of the pig Ia locus.