Eukaryotic mRNA cap binding protein: purification by affinity chromatography on sepharose-coupled m7GDP.
- 1 September 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (9) , 4345-4349
- https://doi.org/10.1073/pnas.76.9.4345
Abstract
A 24,000-dalton polypeptide that binds strongly and can be specifically crosslinked to the 5''-terminal cap structure m7GpppN in eukaryotic mRNA was detected in protein synthesis initiation factor preparations. This polypeptide was purified to apparent homogeneity by 1 chromatographic passage through an affinity resin prepared by coupling the levulinic acid O2'',3''-acetal of m7GDP to AH-Sepharose 4B. Translation, in HeLa [human cervical carcinoma] cell extracts, of capped mRNA including Sindbis virus, reovirus and rabbit globin mRNA was stimulated by the cap-binding protein under conditions that did not increase translation of noncapped RNA of encephalomyocarditis virus and satellite tobacco necrosis virus.This publication has 26 references indexed in Scilit:
- How do eucaryotic ribosomes select initiation regions in messenger RNA?Cell, 1978
- Nonspecific effect of m7GMP on protein-RNA interactions.Journal of Biological Chemistry, 1978
- A polypeptide in eukaryotic initiation factors that crosslinks specifically to the 5'-terminal cap in mRNA.Proceedings of the National Academy of Sciences, 1978
- RNA‐Binding proteins of rabbit reticulocytes contain the two elongation factors and some of the initiation factors of translationFEBS Letters, 1978
- Translational control: recognition of the methylated 5' end and an internal sequence in eukaryotic mRNA by the initiation factor that binds methionyl-tRNAfMet.Proceedings of the National Academy of Sciences, 1978
- Importance of 5'-terminal blocking structure to stabilize mRNA in eukaryotic protein synthesis.Proceedings of the National Academy of Sciences, 1977
- Competition between globin messenger ribonucleic acids for a discriminating initiation factor.Journal of Biological Chemistry, 1977
- 5′-Terminal structure and mRNA stabilityNature, 1977
- Competition between cellular and viral mRNAs in vitro is regulated by a messenger discriminatory initiation factor.Proceedings of the National Academy of Sciences, 1976
- Agarose linked adenosine and guanosine-5′-monophosphate; a new general method for the coupling of ribonucleotides to polymers through their cis-diolsNucleic Acids Research, 1975