Direct evidence that the kinase activity of protein kinase C is involved in transcriptional activation through a TPA‐responsive element
- 1 July 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 252 (1-2) , 144-146
- https://doi.org/10.1016/0014-5793(89)80907-x
Abstract
In order to examine the involvement of protein kinase C (PKC) in the transcriptional activation of genes by TPA (12-O-tetradecanoyl phorbol 13-acetate) we have constructed a series of PKC expression plasmids. Transient expression of an active fragment of PKC in rat fibroblasts resulted in the transcriptional activation of a TRE (TPA-responsive element)-CAT chimeric gene which contains various repetitions of collagenase TREs. These provide the first direct evidence that kinase activity of PKC is involved in TPA-induced transcriptional activation through TRE.Keywords
This publication has 20 references indexed in Scilit:
- Fos and jun: The AP-1 connectionCell, 1988
- The c-fos protein interacts with c-JunAP-1 to stimulate transcription of AP-1 responsive genesCell, 1988
- The Fos Complex and Fos-Related Antigens Recognize Sequence Elements That Co ntain AP-1 Binding SitesScience, 1988
- Human Proto-Oncogene c- jun Encodes a DNA Binding Protein with Structural and Functional Properties of Transcription Factor AP-1Science, 1987
- Purified transcription factor AP-1 interacts with TPA-inducible enhancer elementsCell, 1987
- Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factorCell, 1987
- Tissue-specific expression of three distinct types of rabbit protein kinase CNature, 1987
- Multiple, Distinct Forms of Bovine and Human Protein Kinase C Suggest Diversity in Cellular Signaling PathwaysScience, 1986
- Cloning and expression of multiple protein kinase C cDNAsCell, 1986
- Rat cell line 3y1 and its virogenic polyoma‐ and SV40‐transformed derivativesInternational Journal of Cancer, 1975