Calcitonin increases calcium content and glucose-6-phosphatase activity in hepatic microsomes of rats

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Abstract
The effect of calcitonin (CT) on Ca content and glucose-6-phosphatase activity in the hepatic microsomes of rats was investigated. A single s.c. administration of CT (80 MRC [Metabolic Research Council] mU/100 g body wt) produced a significant increase in microsomal Ca content and a corresponding elevation of microsomal glucose-6-phosphatase activity. An appreciable effect of CT was observed at a dose of 20 MRC mU/100 g body wt. The removal of Ca by 10 mM EGTA [ethylene glycol bis (.beta. aminoethyl ether) N,N,N''N''-tetraacetic acid] washing of the microsomes caused a marked reduction of glucose-6-phosphatase activity raised by CT administration. The addition of Ca ion at the amount of 10-2-103 nmoles Ca2+ per mg protein in the microsomes produced a significant increase in glucose-6-phosphatase activity. Apparently microsomal Ca increased by CT administration activates glucose-6-phosphatase in the hepatic microsomes of rats.