THE COAGULATION DEFECT IN HEMOPHILIA: THE CLOT PROMOTING ACTIVITY IN HEMOPHILIA OF BERKEFELDED NORMAL HUMAN PLASMA FREE FROM FIBRINOGEN AND PROTHROMBIN 1

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Abstract
Using precipitation and adsorption methods it was possible to make preps. from citrated normal plasma free from fibrinogen and prothrombin. After the removal of either prothrombin or both prothrombin and fibrinogen from citrated normal human plasma it was found that the remaining fluid promoted the clotting of hemophilic blood in vitro. When similar preps. were injected intraven. into patients with hemophilia the coagulation time of their blood approached normal limits. Repeated injs. maintained the shortened coagulation time of the patient''s blood. The effects of the injections were entirely similar to those of unmodified normal human plasma it was found that the remaining fluid promoted the clotting of hemophilic blood in vitro. When similar preps. were injected intraven. into patients with hemophilia the coagulation time of their blood approached normal limits. Repeated injs. maintained the shortened coagulation time of the patient''s blood. The effects of the injections were entirely similar to those of unmodified normal human plasma or whole citrated blood.